Source: Bovine pancreas
Appearancewhite crystalline powder
Storage SituationStored in a cool and dry well-closed container. Keep away from moisture and strong light/heat.
Shelf LifeTwo years when properly stored
CharacterWhite or off-white crystalline powder or amorphous powder
IdentificationAppear purple red
Clarity and color of the solutionSolutions should be clear, colorless or almost colorless
Loss on drying≤5.0%
Residue on ignition≤2.5%
Total amount of aerobe≤1000CFU/g
Molds and yeast count≤100CFU/g
Escherichia coliNot detectable
Pseudomonas aeruginosaNot detectable
Staphylococcus aureusNot detectable
Potency determination It contains NLT 1000 USP Chymotrypsin Units/mg, calculated on the dried basis
Chymotrypsin is also called chymotrypsinogen.
Chymotrypsin is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).
Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule.
Chymotrypsin is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9.
Chymotrypsin is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, Chymotrypsin will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority
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